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epsM [2018-09-20 18:43:53]
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epsM [2018-09-20 18:43:53]

similar to serine O-acetyltransferase, extracellular polysaccharide synthesis
Locus
BSU34240
Isoelectric point
9.43
Molecular weight
22.34 kDa
Protein length
216 aa Sequence Blast
Gene length
648 bp Sequence Blast
Function
Biofilm formation, biosynthesis of N,N'-diacetylbacillosamine
Product
unknown
Essential
no
Synonyms
yvfD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Matrix polysaccharide synthesis

    • Gene

    Coordinates
    3,516,233 → 3,516,883

    Phenotypes of a mutant

  • smooth colonies on MsGG medium, no biofilm formation PubMed

    • The protein

    Protein family

  • transferase hexapeptide repeat family (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • RemA: activation, PubMed, in RemA regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in SinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

    • Regulation

  • repressed by SinR PubMed

    • Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed
  • expression is increased in rsiX mutants PubMed
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    Biological materials

    Mutant

  • MGNA-B609 (yvfD::erm), available at the NBRP B. subtilis, Japan
  • BKE34240 (ΔepsM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTCACCCACAATGGCCACAT, downstream forward: _UP4_CAAACATCAAACAAAGGATG
  • BKK34240 (ΔepsM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTCACCCACAATGGCCACAT, downstream forward: _UP4_CAAACATCAAACAAAGGATG

    • Labs working on this gene/protein

  • Richard Losick, Harvard Univ., Cambridge, USA homepage

    • References

    Reviews

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    Research papers

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    =The EAR RNA switch=

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